Journal:

Article Title: Differential Expression of Canonical (Classical) Transient Receptor Potential Channels in Guinea Pig Enteric Nervous System

doi: 10.1002/cne.21874

Figure Lengend Snippet: Primary Antibodies Used in the Study

Article Snippet: The sequence is H-RSESIAFIEESKKD ADEVFSS-NH2 Sheep 1:1,000 Chemicon/AB1529/0703053959 NPY Synthetic NPY peptide conjugated to bovine thyroglobulin Sheep 1:5,000 Chemicon/AB1583/0703053959 Synaptophysin Protein p38 6 from crude fractions of coated vesicles from bovine brain Mouse 1:200 Dako/M0776/00017473 TRPC1 Peptide QLYDK GYTSK EQKDC, corresponding to amino acid residues 557−571 of human TRPC1 (Accession {"type":"entrez-protein","attrs":{"text":"P48995","term_id":"1351302","term_text":"P48995"}} P48995 ) Rabbit 1:100 Alomone/ACC-010/AN-04 TRPC3 Peptide HKLSE KLNPS VLRC, corresponding to residues 822−835 of mouse TRPC3 (Accession {"type":"entrez-protein","attrs":{"text":"Q9QZC1","term_id":"10720320","term_text":"Q9QZC1"}} Q9QZC1 ) Rabbit 1:100 Alomone/ACC-016/AN-03 TRPC4 Peptide (C)KEKH AHEED SSIDY DL, corresponding to residues 943−958 of mouse TRPC4 (Accession {"type":"entrez-protein","attrs":{"text":"Q9QUQ5","term_id":"14548291","term_text":"Q9QUQ5"}} Q9QUQ5 ) Rabbit 1:100 Alomone/ACC-018/AN-01 TRPC5 Peptide (C)HKWGDGQEEQVTTRL corresponding to residues 959−973 of human TRPC5 (Accession {"type":"entrez-protein","attrs":{"text":"Q9UL62","term_id":"10720321","term_text":"Q9UL62"}} Q9UL62 ) Rabbit 1:100 Alomone/ACC-020/AN-07 TRPC6 Peptide (C)RRNE SQDYL LMDELG, corresponding to residues 24−38 of mouse TRPC6 (Accession {"type":"entrez-protein","attrs":{"text":"Q61143","term_id":"342187122","term_text":"Q61143"}} Q61143 ) Rabbit 1:100 Alomone/ACC-017/AN-02 TRPC7 Peptide ILKEL SKEEE DTDSS EEMLA, corresponding to residues 658−677 of human TRPC7 (Accession {"type":"entrez-nucleotide","attrs":{"text":"NM_020389","term_id":"262399373","term_text":"NM_020389"}} NM_020389 ) Rabbit 1:100 Chemicon/AB9326/VR1377347 VIP Synthetic peptide (1−28) coupled to KLH with glutaraldehyde Sheep 1:200 Chemicon/AB1581/0611044826 Open in a separate window Hu, anti-human neuronal protein; ChAT, choline-acetyltransferase; NeuN; neuronal nuclear protein; NOS; nitric oxide synthase; NPY, neuropeptide Y; TRPC, canonical transient receptor potential channel; VIP, vasoactive inhibitory peptide.

Techniques: Recombinant, Purification, Sequencing

Journal:

Article Title: Differential Expression of Canonical (Classical) Transient Receptor Potential Channels in Guinea Pig Enteric Nervous System

doi: 10.1002/cne.21874

Figure Lengend Snippet: TRPC protein expression in the guinea pig ENS. Western blot confirmed the expression of TRPC1 (120kDa), TRPC3 (∼95−100kDa), TRPC4 (∼95−100kDa), and TRPC6 (∼95−100kDa) proteins in LMMP and SMP of the guinea pig ileum, and TRPC1/4/6 proteins in dissociated myenteric ganglia. The immunoblots were at the same level as their corresponding positive controls (i.e., brain lysate). Immunoblots were either completely blocked or significantly reduced by preadsorbing the antibodies with their corresponding blocking peptides.

Article Snippet: The sequence is H-RSESIAFIEESKKD ADEVFSS-NH2 Sheep 1:1,000 Chemicon/AB1529/0703053959 NPY Synthetic NPY peptide conjugated to bovine thyroglobulin Sheep 1:5,000 Chemicon/AB1583/0703053959 Synaptophysin Protein p38 6 from crude fractions of coated vesicles from bovine brain Mouse 1:200 Dako/M0776/00017473 TRPC1 Peptide QLYDK GYTSK EQKDC, corresponding to amino acid residues 557−571 of human TRPC1 (Accession {"type":"entrez-protein","attrs":{"text":"P48995","term_id":"1351302","term_text":"P48995"}} P48995 ) Rabbit 1:100 Alomone/ACC-010/AN-04 TRPC3 Peptide HKLSE KLNPS VLRC, corresponding to residues 822−835 of mouse TRPC3 (Accession {"type":"entrez-protein","attrs":{"text":"Q9QZC1","term_id":"10720320","term_text":"Q9QZC1"}} Q9QZC1 ) Rabbit 1:100 Alomone/ACC-016/AN-03 TRPC4 Peptide (C)KEKH AHEED SSIDY DL, corresponding to residues 943−958 of mouse TRPC4 (Accession {"type":"entrez-protein","attrs":{"text":"Q9QUQ5","term_id":"14548291","term_text":"Q9QUQ5"}} Q9QUQ5 ) Rabbit 1:100 Alomone/ACC-018/AN-01 TRPC5 Peptide (C)HKWGDGQEEQVTTRL corresponding to residues 959−973 of human TRPC5 (Accession {"type":"entrez-protein","attrs":{"text":"Q9UL62","term_id":"10720321","term_text":"Q9UL62"}} Q9UL62 ) Rabbit 1:100 Alomone/ACC-020/AN-07 TRPC6 Peptide (C)RRNE SQDYL LMDELG, corresponding to residues 24−38 of mouse TRPC6 (Accession {"type":"entrez-protein","attrs":{"text":"Q61143","term_id":"342187122","term_text":"Q61143"}} Q61143 ) Rabbit 1:100 Alomone/ACC-017/AN-02 TRPC7 Peptide ILKEL SKEEE DTDSS EEMLA, corresponding to residues 658−677 of human TRPC7 (Accession {"type":"entrez-nucleotide","attrs":{"text":"NM_020389","term_id":"262399373","term_text":"NM_020389"}} NM_020389 ) Rabbit 1:100 Chemicon/AB9326/VR1377347 VIP Synthetic peptide (1−28) coupled to KLH with glutaraldehyde Sheep 1:200 Chemicon/AB1581/0611044826 Open in a separate window Hu, anti-human neuronal protein; ChAT, choline-acetyltransferase; NeuN; neuronal nuclear protein; NOS; nitric oxide synthase; NPY, neuropeptide Y; TRPC, canonical transient receptor potential channel; VIP, vasoactive inhibitory peptide.

Techniques: Expressing, Western Blot, Blocking Assay

Journal:

Article Title: Differential Expression of Canonical (Classical) Transient Receptor Potential Channels in Guinea Pig Enteric Nervous System

doi: 10.1002/cne.21874

Figure Lengend Snippet: Immunohistochemical demonstration of TRPC subunits in the ENS. TRPC subunits were labeled with indocarbocyanine (Cy3, red). The pan neuronal marker HuC/D (Hu) was labeled with fluorescein isothiocyanate (FITC, green). TRPC1 immunoreactivity (IR) was expressed in a subpopulation of neurons in the myenteric (A) and submucosal (B) plexuses. Double-labeling with an antibody for Hu confirmed that all TRPC1-IR cells are immunoreactive for Hu (A’, B’). TRPC3-IR was not found in the myenteric plexus (C, C’), but was expressed in a small neuronal population in submucosal ganglia (D) and was colocalized with Hu-IR (D’). TRPC4-IR was found in neuronal cell bodies and nerve fibers in both the myenteric (E) and submucosal (F) plexuses. TRPC4-immunoreactive nerve cell bodies were colocalized with Hu (E’, F’). TRPC6- IR was found in neuronal cell bodies and nerve fibers in both the myenteric (G) and submucosal (H) plexuses. TRPC6-IR nerve cell bodies were colocalized with Hu-IR (G’, H’). Preadsorption with the corresponding blocking peptide decreased or abolished immunostaining as shown in the example for the submucosal plexus (I-L). BP, blocking peptide. Scale bar = 20 μm for all panels. Magenta-green copy of this figure is available as supplementary figure 2.

Article Snippet: The sequence is H-RSESIAFIEESKKD ADEVFSS-NH2 Sheep 1:1,000 Chemicon/AB1529/0703053959 NPY Synthetic NPY peptide conjugated to bovine thyroglobulin Sheep 1:5,000 Chemicon/AB1583/0703053959 Synaptophysin Protein p38 6 from crude fractions of coated vesicles from bovine brain Mouse 1:200 Dako/M0776/00017473 TRPC1 Peptide QLYDK GYTSK EQKDC, corresponding to amino acid residues 557−571 of human TRPC1 (Accession {"type":"entrez-protein","attrs":{"text":"P48995","term_id":"1351302","term_text":"P48995"}} P48995 ) Rabbit 1:100 Alomone/ACC-010/AN-04 TRPC3 Peptide HKLSE KLNPS VLRC, corresponding to residues 822−835 of mouse TRPC3 (Accession {"type":"entrez-protein","attrs":{"text":"Q9QZC1","term_id":"10720320","term_text":"Q9QZC1"}} Q9QZC1 ) Rabbit 1:100 Alomone/ACC-016/AN-03 TRPC4 Peptide (C)KEKH AHEED SSIDY DL, corresponding to residues 943−958 of mouse TRPC4 (Accession {"type":"entrez-protein","attrs":{"text":"Q9QUQ5","term_id":"14548291","term_text":"Q9QUQ5"}} Q9QUQ5 ) Rabbit 1:100 Alomone/ACC-018/AN-01 TRPC5 Peptide (C)HKWGDGQEEQVTTRL corresponding to residues 959−973 of human TRPC5 (Accession {"type":"entrez-protein","attrs":{"text":"Q9UL62","term_id":"10720321","term_text":"Q9UL62"}} Q9UL62 ) Rabbit 1:100 Alomone/ACC-020/AN-07 TRPC6 Peptide (C)RRNE SQDYL LMDELG, corresponding to residues 24−38 of mouse TRPC6 (Accession {"type":"entrez-protein","attrs":{"text":"Q61143","term_id":"342187122","term_text":"Q61143"}} Q61143 ) Rabbit 1:100 Alomone/ACC-017/AN-02 TRPC7 Peptide ILKEL SKEEE DTDSS EEMLA, corresponding to residues 658−677 of human TRPC7 (Accession {"type":"entrez-nucleotide","attrs":{"text":"NM_020389","term_id":"262399373","term_text":"NM_020389"}} NM_020389 ) Rabbit 1:100 Chemicon/AB9326/VR1377347 VIP Synthetic peptide (1−28) coupled to KLH with glutaraldehyde Sheep 1:200 Chemicon/AB1581/0611044826 Open in a separate window Hu, anti-human neuronal protein; ChAT, choline-acetyltransferase; NeuN; neuronal nuclear protein; NOS; nitric oxide synthase; NPY, neuropeptide Y; TRPC, canonical transient receptor potential channel; VIP, vasoactive inhibitory peptide.

Techniques: Immunohistochemical staining, Labeling, Marker, Blocking Assay, Immunostaining

Journal:

Article Title: Differential Expression of Canonical (Classical) Transient Receptor Potential Channels in Guinea Pig Enteric Nervous System

doi: 10.1002/cne.21874

Figure Lengend Snippet: Canonical transient receptor potential channel (TRPC) mRNA expression in the guinea pig enteric nervous system (ENS). RT-PCR analysis with primers specific for each of the TRPC subunits identified mRNA transcripts for TRPC1 through TRPC7 in the guinea pig brain (i.e., positive controls). Transcripts encoding TRPC1/3/4/6/7 were expressed in the submucosal plexus (SMP). Bands representing TRPC2/5 were not detected in the SMP preparations. Transcripts encoding TRPC1 to TRPC7 were all observed in the longitudinal muscle-myenteric plexus (LMMP) preparations, while transcripts encoding TRPC1 to TRPC6 were detected also in dissociated myenteric ganglia. Sizes of the bands matched the expected lengths of the amplified fragments of each TRPC subunit (TRPC1, 400 bp; TRPC2, 370 bp; TRPC3, 340 bp; TRPC4, 370 bp; TRPC5, 484 bp; TRPC6 327 bp; TRPC7 429 bp).

Article Snippet: The sequence is H-RSESIAFIEESKKD ADEVFSS-NH2 Sheep 1:1,000 Chemicon/AB1529/0703053959 NPY Synthetic NPY peptide conjugated to bovine thyroglobulin Sheep 1:5,000 Chemicon/AB1583/0703053959 Synaptophysin Protein p38 6 from crude fractions of coated vesicles from bovine brain Mouse 1:200 Dako/M0776/00017473 TRPC1 Peptide QLYDK GYTSK EQKDC, corresponding to amino acid residues 557−571 of human TRPC1 (Accession {"type":"entrez-protein","attrs":{"text":"P48995","term_id":"1351302","term_text":"P48995"}} P48995 ) Rabbit 1:100 Alomone/ACC-010/AN-04 TRPC3 Peptide HKLSE KLNPS VLRC, corresponding to residues 822−835 of mouse TRPC3 (Accession {"type":"entrez-protein","attrs":{"text":"Q9QZC1","term_id":"10720320","term_text":"Q9QZC1"}} Q9QZC1 ) Rabbit 1:100 Alomone/ACC-016/AN-03 TRPC4 Peptide (C)KEKH AHEED SSIDY DL, corresponding to residues 943−958 of mouse TRPC4 (Accession {"type":"entrez-protein","attrs":{"text":"Q9QUQ5","term_id":"14548291","term_text":"Q9QUQ5"}} Q9QUQ5 ) Rabbit 1:100 Alomone/ACC-018/AN-01 TRPC5 Peptide (C)HKWGDGQEEQVTTRL corresponding to residues 959−973 of human TRPC5 (Accession {"type":"entrez-protein","attrs":{"text":"Q9UL62","term_id":"10720321","term_text":"Q9UL62"}} Q9UL62 ) Rabbit 1:100 Alomone/ACC-020/AN-07 TRPC6 Peptide (C)RRNE SQDYL LMDELG, corresponding to residues 24−38 of mouse TRPC6 (Accession {"type":"entrez-protein","attrs":{"text":"Q61143","term_id":"342187122","term_text":"Q61143"}} Q61143 ) Rabbit 1:100 Alomone/ACC-017/AN-02 TRPC7 Peptide ILKEL SKEEE DTDSS EEMLA, corresponding to residues 658−677 of human TRPC7 (Accession {"type":"entrez-nucleotide","attrs":{"text":"NM_020389","term_id":"262399373","term_text":"NM_020389"}} NM_020389 ) Rabbit 1:100 Chemicon/AB9326/VR1377347 VIP Synthetic peptide (1−28) coupled to KLH with glutaraldehyde Sheep 1:200 Chemicon/AB1581/0611044826 Open in a separate window Hu, anti-human neuronal protein; ChAT, choline-acetyltransferase; NeuN; neuronal nuclear protein; NOS; nitric oxide synthase; NPY, neuropeptide Y; TRPC, canonical transient receptor potential channel; VIP, vasoactive inhibitory peptide.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification

Journal:

Article Title: Differential Expression of Canonical (Classical) Transient Receptor Potential Channels in Guinea Pig Enteric Nervous System

doi: 10.1002/cne.21874

Figure Lengend Snippet: TRPC6-IR nerve fibers in myenteric and submucosal plexuses. A: TRPC6-IR varicose nerve fibers in myenteric ganglia and in interganglionic fiber bundles. Within some myenteric ganglia, TRPC6-IR fibers form complexes that encircle individual ganglionic cell somas. B: TRPC6-IR nerve fibers were present in the tertiary division of the myenteric plexus. C: TRPC6-IR nerve cell bodies and varicose nerve fibers in submucosal ganglia and in interganglionic connectives. D: TRPC6-IR nerve fibers in the tertiary division of the submucosal plexus. E: TRPC6-IR nerve fibers in the circular muscle plexus. F: TRPC6-IR nerve fibers around submucosal blood vessels. G-H: Paired images showing colocalization of TRPC6-IR with synaptophysin-IR in the circular muscle plexus. The arrows point to nerve fibers that coexpress TRPC6- and synaptophysin-IR. Scale bar = 20 μm for all panels.

Article Snippet: The sequence is H-RSESIAFIEESKKD ADEVFSS-NH2 Sheep 1:1,000 Chemicon/AB1529/0703053959 NPY Synthetic NPY peptide conjugated to bovine thyroglobulin Sheep 1:5,000 Chemicon/AB1583/0703053959 Synaptophysin Protein p38 6 from crude fractions of coated vesicles from bovine brain Mouse 1:200 Dako/M0776/00017473 TRPC1 Peptide QLYDK GYTSK EQKDC, corresponding to amino acid residues 557−571 of human TRPC1 (Accession {"type":"entrez-protein","attrs":{"text":"P48995","term_id":"1351302","term_text":"P48995"}} P48995 ) Rabbit 1:100 Alomone/ACC-010/AN-04 TRPC3 Peptide HKLSE KLNPS VLRC, corresponding to residues 822−835 of mouse TRPC3 (Accession {"type":"entrez-protein","attrs":{"text":"Q9QZC1","term_id":"10720320","term_text":"Q9QZC1"}} Q9QZC1 ) Rabbit 1:100 Alomone/ACC-016/AN-03 TRPC4 Peptide (C)KEKH AHEED SSIDY DL, corresponding to residues 943−958 of mouse TRPC4 (Accession {"type":"entrez-protein","attrs":{"text":"Q9QUQ5","term_id":"14548291","term_text":"Q9QUQ5"}} Q9QUQ5 ) Rabbit 1:100 Alomone/ACC-018/AN-01 TRPC5 Peptide (C)HKWGDGQEEQVTTRL corresponding to residues 959−973 of human TRPC5 (Accession {"type":"entrez-protein","attrs":{"text":"Q9UL62","term_id":"10720321","term_text":"Q9UL62"}} Q9UL62 ) Rabbit 1:100 Alomone/ACC-020/AN-07 TRPC6 Peptide (C)RRNE SQDYL LMDELG, corresponding to residues 24−38 of mouse TRPC6 (Accession {"type":"entrez-protein","attrs":{"text":"Q61143","term_id":"342187122","term_text":"Q61143"}} Q61143 ) Rabbit 1:100 Alomone/ACC-017/AN-02 TRPC7 Peptide ILKEL SKEEE DTDSS EEMLA, corresponding to residues 658−677 of human TRPC7 (Accession {"type":"entrez-nucleotide","attrs":{"text":"NM_020389","term_id":"262399373","term_text":"NM_020389"}} NM_020389 ) Rabbit 1:100 Chemicon/AB9326/VR1377347 VIP Synthetic peptide (1−28) coupled to KLH with glutaraldehyde Sheep 1:200 Chemicon/AB1581/0611044826 Open in a separate window Hu, anti-human neuronal protein; ChAT, choline-acetyltransferase; NeuN; neuronal nuclear protein; NOS; nitric oxide synthase; NPY, neuropeptide Y; TRPC, canonical transient receptor potential channel; VIP, vasoactive inhibitory peptide.

Techniques:

Journal:

Article Title: Differential Expression of Canonical (Classical) Transient Receptor Potential Channels in Guinea Pig Enteric Nervous System

doi: 10.1002/cne.21874

Figure Lengend Snippet: Neurochemical coding of TRPC6-immunoreactive neurons in the myenteric plexus of the guinea pig ileum. TRPC6 IR was colocalized with ChAT (A, A’) and NOS (C, C’), but was not colocalized with calretinin (B, B’) nor calbindin (D, D’). Arrows indicate neurons that are positive for TRPC6 as well as neurochemical markers. Arrowheads indicate neurons that are positive for TRPC6 only. Scale bar = 20 μm for all panels.

Article Snippet: The sequence is H-RSESIAFIEESKKD ADEVFSS-NH2 Sheep 1:1,000 Chemicon/AB1529/0703053959 NPY Synthetic NPY peptide conjugated to bovine thyroglobulin Sheep 1:5,000 Chemicon/AB1583/0703053959 Synaptophysin Protein p38 6 from crude fractions of coated vesicles from bovine brain Mouse 1:200 Dako/M0776/00017473 TRPC1 Peptide QLYDK GYTSK EQKDC, corresponding to amino acid residues 557−571 of human TRPC1 (Accession {"type":"entrez-protein","attrs":{"text":"P48995","term_id":"1351302","term_text":"P48995"}} P48995 ) Rabbit 1:100 Alomone/ACC-010/AN-04 TRPC3 Peptide HKLSE KLNPS VLRC, corresponding to residues 822−835 of mouse TRPC3 (Accession {"type":"entrez-protein","attrs":{"text":"Q9QZC1","term_id":"10720320","term_text":"Q9QZC1"}} Q9QZC1 ) Rabbit 1:100 Alomone/ACC-016/AN-03 TRPC4 Peptide (C)KEKH AHEED SSIDY DL, corresponding to residues 943−958 of mouse TRPC4 (Accession {"type":"entrez-protein","attrs":{"text":"Q9QUQ5","term_id":"14548291","term_text":"Q9QUQ5"}} Q9QUQ5 ) Rabbit 1:100 Alomone/ACC-018/AN-01 TRPC5 Peptide (C)HKWGDGQEEQVTTRL corresponding to residues 959−973 of human TRPC5 (Accession {"type":"entrez-protein","attrs":{"text":"Q9UL62","term_id":"10720321","term_text":"Q9UL62"}} Q9UL62 ) Rabbit 1:100 Alomone/ACC-020/AN-07 TRPC6 Peptide (C)RRNE SQDYL LMDELG, corresponding to residues 24−38 of mouse TRPC6 (Accession {"type":"entrez-protein","attrs":{"text":"Q61143","term_id":"342187122","term_text":"Q61143"}} Q61143 ) Rabbit 1:100 Alomone/ACC-017/AN-02 TRPC7 Peptide ILKEL SKEEE DTDSS EEMLA, corresponding to residues 658−677 of human TRPC7 (Accession {"type":"entrez-nucleotide","attrs":{"text":"NM_020389","term_id":"262399373","term_text":"NM_020389"}} NM_020389 ) Rabbit 1:100 Chemicon/AB9326/VR1377347 VIP Synthetic peptide (1−28) coupled to KLH with glutaraldehyde Sheep 1:200 Chemicon/AB1581/0611044826 Open in a separate window Hu, anti-human neuronal protein; ChAT, choline-acetyltransferase; NeuN; neuronal nuclear protein; NOS; nitric oxide synthase; NPY, neuropeptide Y; TRPC, canonical transient receptor potential channel; VIP, vasoactive inhibitory peptide.

Techniques:

Journal:

Article Title: Differential Expression of Canonical (Classical) Transient Receptor Potential Channels in Guinea Pig Enteric Nervous System

doi: 10.1002/cne.21874

Figure Lengend Snippet: Neurochemical coding of TRPC6-IR in the submucosal plexus. TRPC6-IR was exclusively colocalized with VIP (D, D’). No colocalization of TRPC6 with ChAT (A, A’), calretinin (B, B’), NPY (C, C’), calbindin (E, E’) nor NeuN (F, F’) was found. Arrows indicate neurons that are positive for TRPC6 as well as neurochemical markers. Arrowheads indicate neurons that are positive for TRPC6 only. Scale bar = 20 μm for all panels.

Article Snippet: The sequence is H-RSESIAFIEESKKD ADEVFSS-NH2 Sheep 1:1,000 Chemicon/AB1529/0703053959 NPY Synthetic NPY peptide conjugated to bovine thyroglobulin Sheep 1:5,000 Chemicon/AB1583/0703053959 Synaptophysin Protein p38 6 from crude fractions of coated vesicles from bovine brain Mouse 1:200 Dako/M0776/00017473 TRPC1 Peptide QLYDK GYTSK EQKDC, corresponding to amino acid residues 557−571 of human TRPC1 (Accession {"type":"entrez-protein","attrs":{"text":"P48995","term_id":"1351302","term_text":"P48995"}} P48995 ) Rabbit 1:100 Alomone/ACC-010/AN-04 TRPC3 Peptide HKLSE KLNPS VLRC, corresponding to residues 822−835 of mouse TRPC3 (Accession {"type":"entrez-protein","attrs":{"text":"Q9QZC1","term_id":"10720320","term_text":"Q9QZC1"}} Q9QZC1 ) Rabbit 1:100 Alomone/ACC-016/AN-03 TRPC4 Peptide (C)KEKH AHEED SSIDY DL, corresponding to residues 943−958 of mouse TRPC4 (Accession {"type":"entrez-protein","attrs":{"text":"Q9QUQ5","term_id":"14548291","term_text":"Q9QUQ5"}} Q9QUQ5 ) Rabbit 1:100 Alomone/ACC-018/AN-01 TRPC5 Peptide (C)HKWGDGQEEQVTTRL corresponding to residues 959−973 of human TRPC5 (Accession {"type":"entrez-protein","attrs":{"text":"Q9UL62","term_id":"10720321","term_text":"Q9UL62"}} Q9UL62 ) Rabbit 1:100 Alomone/ACC-020/AN-07 TRPC6 Peptide (C)RRNE SQDYL LMDELG, corresponding to residues 24−38 of mouse TRPC6 (Accession {"type":"entrez-protein","attrs":{"text":"Q61143","term_id":"342187122","term_text":"Q61143"}} Q61143 ) Rabbit 1:100 Alomone/ACC-017/AN-02 TRPC7 Peptide ILKEL SKEEE DTDSS EEMLA, corresponding to residues 658−677 of human TRPC7 (Accession {"type":"entrez-nucleotide","attrs":{"text":"NM_020389","term_id":"262399373","term_text":"NM_020389"}} NM_020389 ) Rabbit 1:100 Chemicon/AB9326/VR1377347 VIP Synthetic peptide (1−28) coupled to KLH with glutaraldehyde Sheep 1:200 Chemicon/AB1581/0611044826 Open in a separate window Hu, anti-human neuronal protein; ChAT, choline-acetyltransferase; NeuN; neuronal nuclear protein; NOS; nitric oxide synthase; NPY, neuropeptide Y; TRPC, canonical transient receptor potential channel; VIP, vasoactive inhibitory peptide.

Techniques: